Fluorescence signal of calcein collected from tumor t issue and quadriceps muscle was analyzed using a fluorescence plate reader Synergy HT Multi Mod e Microplate Reader, Biotek, Winooski, VT and compared with each group. Open square with error bar represents experimental data by in vitro optical density measurements and dotted line is fitting curves by Gompertz function. Continuous photohemolysis CPH measurement is assumed that photohemolysis is attributed to cooperative photochemical and thermal reactions simultaneously. In , EPR effect was described by Matsumura et al. Knock in group had the most enhanced accumulation rate compared with the other murine models and it was significantly different f rom C57 and Berkeley groups.
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